COMPLEMENTATION BETWEEN ad-5/7 ALLELES IN YEAST

Complementation between Ad-5/7 Alleles in Yeast.

NTRAGENIC complementation occurs when a full or partial restoration of 'nonmutant phenotype results from the association of two different mutant alleles in a heterozygote, heterocaryon, or heterogenote. It has been demonstrated for a number of gene loci in a variety of microorganisms which include fungi. (FINCHAM and PATEMAN 1957; GILES, PARTRIDGE and NELSON 1957; CATCHESIDE and OVERTON 1958), ...

Complementation between alleles of the Td locus in Neurospora crassa.

Genetic complementation in yeast reveals functional similarities between the catalytic subunits of mammalian signal peptidase complex.

Type I signal peptidases (SPs) comprise a family of structurally related enzymes that cleave signal peptides from precursor proteins following their transport out of the cytoplasmic space in eukaryotic and prokaryotic cells. One such enzyme, the mitochondrial inner membrane peptidase, has two catalytic subunits, which recognize distinct cleavage site motifs in their signal peptide substrates. T...

Complementation of soluble phosphofructokinase activity in yeast mutants.

We describe here the genetic and biochemical analyses of two classes of mutations in the soluble phosphofructokinase (PFK I) of Saccharomyces cerevisiae: those leading to the loss of activity and those giving rise to a kinetically altered enzyme. Complementation and allele-testing between these two classes of mutants show that loss of enzyme activity in vitro can come about not only by mutation...

Antimutator Alleles of Yeast DNA Polymerase Gamma Modulate the Balance between DNA Synthesis and Excision

Mutations in mitochondrial DNA (mtDNA) are an important cause of disease and perhaps aging in human. DNA polymerase gamma (pol γ), the unique replicase inside mitochondria, plays a key role in the fidelity of mtDNA replication through selection of the correct nucleotide and 3'-5' exonuclease proofreading. For the first time, we have isolated and characterized antimutator alleles in the yeast po...